A Pure Preparation of the Staphylococcal Typing Bacteriophage 42 D

A pure preparation of the staphylococcal typing bacteriophage-42-D.

The staphylococcal typing-phage 42 D is propagated on bacteria which are lysogenic for the phage 1363, and the latter phage is present as a contaminant in preparations of phage 42D. This contamination can be avoided by propagating a pure clone of phage 42D on non-lysogenic organisms. Such nonlysogenic propagating strains can be isolated after the lysogenic propagating organisms have been irradi...

The Preparation of Relatively Pure Bacteriophage

The method described above, based on the electrophoretic migration of bacteriophage particles into an agar gel and their subsequent re-suspension in a suitable medium, has the following advantages: It is simple and can be readily carried out on a comparatively large scale by merely inserting additional units between the same electrode cups. It requires but one extraction and the resulting phage...

Rapid Method of Determining Coagulase Activity during Staphylococcal Bacteriophage Typing.

The plate test, a modification of the slide test described by Cadness-Graves was developed for the rapid identification of coagulase-positive staphylococci in conjunction with bacteriophage typing. An evaluation of 1,145 cultures by three coagulase-determination methods, the slide, tube, and plate tests, indicates that the plate test is as accurate as the slide tests, and the plate test agrees ...

The Complement of a D-Tree is Pure Shellable

Bacteriophage Typing of the Staphylococci.

INTRODUCTION ...................................................................... 253 TYPING PHAGES...................................................................... 255 TECHNIQUES OF PHAGE PROPAGATION ........................................................... 257 STERILIZATION AND PRESERVATION OF LYSATES ................................................. 258 TECHNIQUE OF PHAGE TYPING.......